Enzimologia - DM 270 (MFN0248)
MATERIALE DIDATTICO
AA 2022/2023
Altri files
AA 2020/2021
Altri files
Esercitazioni
Lezioni
- 0. Background biochemistry for Enzymology
- 00. Course Presentation 2021
- 01. Introduction to Enzymes and NAD 2021
- 02. Redox FAD 2
- 03. Quinone redox 2021
- 04. vitamin C and pterin redox 2021
- 05. Transferases B1 B5 2021
- 06. class 2 B6 and Folate 2021
- 07. Lyases 2021
- 08. Isomerases 2021
- 09. Ligases 2021
- 10. Enzyme intro 2021
- 11. Techniques in enzymology, stopped flow, ITC, isotope use, FTIR 2021
AA 2019/2020
Altri files
AA 2018/2019
Altri files
Articoli
- Computational Design of an Enzyme Catalyst for a Stereoselective Bimolecular Diels-Alder Reaction
- Computational Study of a Model System of Enzyme-Mediated [4+2] Cycloaddition Reaction
- Industrial biotransformations in the synthesis of building blocks leading to drug synthesis
- Nature’s Strategy for Catalyzing Diels-Alder Reaction
- Paper 1: FAAH
- Paper 2: FAAH
- paper 3: FAAH
- Paper on Asp HIV-1 protease
- paper on elastase and oxyanion hole evidence (Tamada 2009 JACS)
- Smith 2008 serine esterase paper
Dispense
Esercitazioni
Lezioni
- 00. Background biochemistry for enzymology
- 01. Introduction, Enzyme classification and NAD
- 02. Class I cofactors: flavin and quinone
- 03. Class I cofactors: vit C and B8 (pterin)
- 04. Class 2 cofactors: pantotenic acid and CoA
- 05. Class 2 cofactors: PLP, FH4 and B12
- 05_1_Class 3: short overview on proteases
- 05_2 Class 3: short overwiev on nucleases mechanism
- 06. Class 4 cofactors: PLP, TPP
- 07. Class 5 and 6 cofactors: PLP, B12, Biotin (vit H)
- 08. Energy compounds and class 6 enzymes
- 09. Enzyme mechanism introduction
- 10. Stopped flow and ITC applied to enzyme studies
- 11 bis. Activity based protein profiling of class 3 enzymes including FAAH
- 11. proteases mechanism: ser and asp proteases
- 12. Non heme iron oxygenases: MMOX, TMO, PHO, C1,2DOX
- 13. Hydrogenases: structure and mechanism
- 14 Hydrogenases: detail of mechanism and mutagenesis
- 15. Oxygen resistant hydrogenase and Hinact state
- 16. FDH e CODH
AA 2017/2018
Altri files
Articoli
- Bacterial lipases
- Di Cera 2009 paper on serine proteases
- Hedstrom 202 serine protease mechanism and specificity
- Hydroiases in non conventional media
- Hydrolases: relevance for organic chemistry
- Idrogenasi. Lubitz 2014 review
- Idrogenasi: Caserta et al 2018
- Idrogenasi: Sommer et al 2018
- Idrogenasi: Wittkamp 2018 review
- Industrial biotransformations in the synthesis of building blocks leading to drug synthesis
- lipases and phospho lipases
- Paper 1: FAAH
- Paper 2: FAAH
- paper 3: FAAH
- Paper on Asp HIV-1 protease
- paper on elastase and oxyanion hole evidence (Tamada 2009 JACS)
- Smith 2008 serine esterase paper
- Solvent tolerant lipases
- Thermoalkaline lipases
Dispense
Esercitazioni
Lezioni
- 0. Background biochemistry concepts for enzymology
- 01. Introduction and Class I enzymes cofactors: NAD(P)+
- 02. Class I cofactors: flavin and quinone
- 03. Class I cofactors: vit C and B8 (pterin)
- 04. Class 2 cofactors: pantotenic acid and CoA
- 05_1_Class 3: short overview on proteases
- 05_2 Class 3: short overwiev on nucleases mechanism
- 06. Class 4 cofactors: PLP, TPP
- 07. Class 5 and 6 cofactors: PLP, B12, Biotin (vit H)
- 08. Energy compounds and class 6 enzymes
- 09. Introduction to enzyme reaction mechanism
- 10. Stopped flow and ITC applied to enzyme studies
- 11 bis. Activity based protein profiling of class 3 enzymes including FAAH
- 11. proteases mechanism: ser and asp proteases
- 12. Non heme iron oxygenases: MMOX, TMO, PHO, C1,2DOX
- 13. Hydrogenases: structure and mechanism
- 14 Hydrogenases: detail of mechanism and mutagenesis
- 15. protein structure of oxygen stable FeFe hydrogenases
AA 2016/2017
Articoli
Dispense
Esercitazioni
Lezioni
- 0. Background biochemistry concepts for enzymology
- 01. Introduction and Class I enzymes cofactors: NAD(P)+
- 02. Class I cofactors: flavin and quinone
- 03. Class I cofactors: vit C and B8 (pterin)
- 04. Class 2 cofactors: pantotenic acid and CoA
- 05_1_Class 3: short overview on proteases
- 05_2 Class 3: short overwiev on nucleases mechanism
- 06. Class 4 cofactors: PLP, TPP
- 07. Class 5 and 6 cofactors: PLP, B12, Biotin (vit H)
- 08. Energy compounds and class 6 enzymes
- 09. Introduction to enzyme reaction mechanism
- 11. proteases mechanism: ser and asp proteases
- 12. Non heme iron oxygenases: MMOX, TMO, PHO, C1,2DOX
- 13. Hydrogenases: structure and mechanism
- 14 Hydrogenases: detail of mechanism and mutagenesis
- 15. protein structure of oxygen stable FeFe hydrogenases